MDA-kb2细胞，人乳腺细胞

产品型号：CRL-2713
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细胞原代牛、狗、鸡、鸭、鱼原代细胞羊原代细胞猪原代细胞兔原代细胞小鼠原代细胞大鼠原代细胞人原代细胞培养基原代细胞冻存液 ATCC 人乳腺癌猪正常细胞猪睾丸细胞系人胶质瘤细胞人成骨细胞人永生化肝细胞人肝癌细胞人卵巢癌细胞人结直肠腺癌人经典小细胞肺癌细胞人小细胞肺癌细胞 Kaiyun官方体育人鼻腔上皮细胞中国仓鼠卵巢悬浮细胞嗜性逆转录病毒包装稳定ky体育官方下载小鼠神经细胞瘤人眼脉洛膜黑色素瘤 PT67 逆转录酶病毒包装细胞鸭子胚胎成纤维细胞猪肾细胞系昆虫细胞猫肾细胞恒河猴胚肾细胞仓鼠卵巢细胞负鼠肾细胞仓鼠肺细胞果蝇胚胎细胞家猫肺成纤维样细胞鸡胚细胞豚鼠心肌来源细胞豚鼠肺成纤维样细胞豚鼠皮肤成纤维样细胞狗肾细胞非洲绿猴胚胎肾上皮细胞猴胚胎肾上皮细胞长臂猿淋巴瘤新生牛眼囊成纤维细胞黑化大家鼠肺成纤维样细胞俄勒冈地鼠细胞家兔皮肤成纤维样细胞昆虫卵巢细胞人胸膜瘤细胞非洲绿猴肾细胞 VERO 衍生株（SVP）白化金黄地鼠肺成纤维样细胞黄胸鼠肺成纤维样细胞白鲢尾鳍细胞蝙蝠肺细胞草鱼肾细胞系大黄鱼肾细胞猴肾细胞系犬肾细胞系人结肠直肠腺癌猪肺泡巨噬细胞 PFHSA05（TLL2）人重组特洛德样蛋白因子 PFHSA04 （BMP1）人重组骨诱导因子人乳腺癌细胞结肠癌细胞成纤维细胞人胚肾细胞动物卵巢细胞动物胚胎细胞动物肺细胞肋骨来源细胞成纤维样细胞动物上皮细胞动物肝脏细胞可诱导表达稳定ky体育官方下载动物气管细胞动物肾细胞动物巨噬细胞人正常细胞人肿瘤细胞、癌细胞小鼠正常细胞小鼠肿瘤细胞、癌细胞大鼠正常细胞大鼠肿瘤细胞其它动物细胞 Kaiyun官方体育

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MDA-kb2细胞，人乳腺细胞

ATCC^® Number:

CRL-2713™

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Designations:	MDA-kb2
Depositors:	VS Wilson
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: mammary gland; breast Cell Type: epithelial
Cellular Products:	luciferase ( [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors (GR) and androgen receptors (AR).)
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MDA-kb2细胞，人乳腺细胞
Isolation:	Isolation date: November 25, 1998
Applications:	assay of androgen receptor ( [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.)
Receptors:	androgen receptor [57526] glucocorticoid receptor, positive [57526]
DNA Profile (STR):	Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 11 D7S820: 10 THO1: 6 TPOX: 10 vWA: 17,18
Age:	48 years adult
Gender:	female
Ethnicity:	Caucasian
Comments:	Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100%
	parental cell line:ATCC HTB-131
References:	57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974

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